names(list_of_cents)
[1] "fold_change_aritmetic" "fold_change_geometric" "fold_change_quadratic" "fold_change_harmonic" "delta_aritmetic" "delta_geometric" "delta_quadratic" "delta_harmonic"
Density plot of centralities
plot_density <- function(df, titulo ){cut = 0.01
df %>% gather() -> df_gathered
df_gathered['value'] %>% filter(abs(value) > cut) %>%
ggplot( aes(x=value)) +
geom_density(fill="deepskyblue4", color="azure4", alpha=.8)+
theme(legend.position="top") +
ylab("Density") + ggtitle(titulo) +
xlab("Centrality") + geom_vline(xintercept=0, linetype="dashed", color = "red", size=.4) -> Density_plot_of_centralities
return(Density_plot_of_centralities)}
map2(list_of_cents, names(list_of_cents), plot_density)
$fold_change_aritmetic
$fold_change_geometric
$fold_change_quadratic
$fold_change_harmonic
$delta_aritmetic
$delta_geometric
$delta_quadratic
$delta_harmonic
#centralities <- Results[,!is.na(as.numeric(colnames(Results)))] %>% gather()
Density_plot_of_centralities.panel <- ggarrange( NULL,Density_plot_of_centralities, nrow = 2, heights= c(1, .4), labels = c('a','b'),
hjust = -0.1, vjust = .8)
Density_plot_of_centralities.panel
fba_list_of_cents
$fold_change_aritmetic
$fold_change_geometric
$fold_change_quadratic
$fold_change_harmonic
$delta_aritmetic
$delta_geometric
$delta_quadratic
$delta_harmonic
NA
Hierarchical clustering
library(tidyverse)
library(scales)
assign_node_type <- function(df){
data <- df %>% rownames_to_column('ID') %>% filter(!str_detect(ID, regex('AA_|AA2', ignore_case = F))) %>%
mutate(`Node type` = ifelse(str_detect(Reaction, regex('\\[[a-z]A\\]', ignore_case = T)), 'Astrocyte', NA)) %>%
mutate(`Node type` = ifelse(str_detect(Reaction, regex('\\[[a-z]N\\]', ignore_case = T)), 'Neuron', `Node type`)) %>%
mutate(`Node type` = ifelse( str_detect(Reaction, regex('\\[[a-z]A\\]|\\[[a-z]N\\]',ignore_case = T), negate = T), 'Exchange', `Node type`)) %>%
mutate(`Node type` = ifelse(str_detect(Reaction, regex('\\[[a-z]A\\]',ignore_case = T)) &
str_detect(Name, regex('DM_|Demand|sink',ignore_case = T)), 'Sink/Demand Astro', `Node type`))%>%
mutate(`Node type` = ifelse(str_detect(Reaction, regex('\\[[a-z]N\\]',ignore_case = T)) &
str_detect(Name, regex('DM_|Demand|sink',ignore_case = T)), 'Sink/Demand Neuron', `Node type`))
return(data)}
map(fba_list_of_cents, assign_node_type) -> data
clean_centralities <- function(df){ df %>% select(-c ("ID", "Name", "Reaction", "Flux", "Sensitivity","Node type") )}
map(data, clean_centralities) -> only_centralities
map( data,get_summaryzed.optimality)
$fold_change_aritmetic
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[ reached getOption("max.print") -- omitted 53 rows ]
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[ reached getOption("max.print") -- omitted 53 rows ]
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[ reached getOption("max.print") -- omitted 53 rows ]
$fold_change_harmonic
[,1]
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[ reached getOption("max.print") -- omitted 53 rows ]
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[ reached getOption("max.print") -- omitted 53 rows ]
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[ reached getOption("max.print") -- omitted 53 rows ]
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[928,] 5.802306e-17
[929,] 9.283689e-16
[930,] 3.024742e-01
[931,] 2.417627e-17
[932,] 1.535427e-01
[933,] 4.083400e-01
[934,] 1.535427e-01
[935,] 1.824995e-01
[936,] 3.771499e-16
[937,] 1.434756e-01
[938,] 1.434756e-01
[939,] 2.376135e-01
[940,] 2.417627e-17
[941,] 2.376135e-01
[942,] 2.417627e-17
[943,] 5.318780e-17
[944,] 0.000000e+00
[945,] 0.000000e+00
[946,] 8.581239e-01
[947,] 0.000000e+00
[948,] 1.824995e-01
[949,] 1.653657e-15
[950,] 2.562685e-16
[951,] 4.706308e-16
[952,] 2.662552e-01
[953,] 0.000000e+00
[954,] 1.015404e-16
[955,] 5.947363e-16
[956,] 7.736408e-17
[957,] 0.000000e+00
[958,] 1.566623e-15
[959,] 0.000000e+00
[960,] 6.672652e-16
[961,] 1.535427e-01
[962,] 2.898834e-32
[963,] 0.000000e+00
[964,] 7.736408e-17
[965,] 1.535427e-01
[966,] 1.535427e-01
[967,] 2.376135e-01
[968,] 9.670510e-18
[969,] 4.083400e-01
[970,] 2.898834e-32
[971,] 0.000000e+00
[972,] 0.000000e+00
[973,] 2.852800e-16
[974,] 2.417627e-17
[975,] 0.000000e+00
[976,] 1.934102e-17
[977,] 7.736408e-16
[978,] 2.007721e-15
[979,] 1.392553e-15
[980,] 1.450576e-17
[981,] 2.439427e-15
[982,] 9.573805e-16
[983,] 1.208814e-16
[984,] 4.083400e-01
[985,] 1.535427e-01
[986,] 1.535427e-01
[987,] 0.000000e+00
[988,] 0.000000e+00
[989,] 7.736408e-17
[990,] 0.000000e+00
[991,] 0.000000e+00
[992,] 0.000000e+00
[993,] 4.083400e-01
[994,] 0.000000e+00
[995,] 0.000000e+00
[996,] 0.000000e+00
[997,] 1.535427e-01
[998,] 1.434756e-01
[999,] 1.450576e-17
[1000,] 7.736408e-17
[ reached getOption("max.print") -- omitted 53 rows ]
heatmap
dim(my.corr.matrices$fold_change_aritmetic)
[1] 1053 1053
my.corr.matrices$delta_aritmetic -> my.corr.mat
left_annotation <- rowAnnotation(`Nodes` = node_types, col = list( `Nodes` = c(
"Astrocyte" = "deepskyblue3",
'Exchange' = 'darkgreen',
'Sink/Demand Astro'='darkgoldenrod',
"Neuron" = "brown2",
'Sink/Demand Neuron'= 'blueviolet' )))
total.abs.centrality$fold_change_aritmetic -> a_total.abs.cent
summaryzed.optimality$fold_change_aritmetic -> a_summaryzed.opti
right_annotation <-
rowAnnotation(gap = unit(12, "points"),Ce = anno_barplot(bar_width = 0.01,width = unit(1.5, "cm"), border = T,a_total.abs.cent, gp = gpar(col = 'azure4')),
Op = anno_barplot(bar_width = 0.01,width = unit(1.5, "cm") ,border = T, a_summaryzed.opti, gp = gpar(col = 'azure4')))
ht <- Heatmap(my.corr.mat, name = "Correlation", left_annotation =left_annotation,
right_annotation=right_annotation,
clustering_distance_columns = function(m) dist(m, method = 'euclidean'),
cluster_columns = function(x) fastcluster::hclust(dist(x), "average"),
clustering_distance_rows = function(m) dist(m, method = 'euclidean'),
cluster_rows = function(x) fastcluster::hclust(dist(x), "average"),
row_km = 2,
column_km = 2,
# row_split = paste0("cluster ", pa$clustering),
# column_split = paste0("cluster ", pa$clustering),
border = TRUE,
row_dend_width = unit(3, "cm"),
row_gap = unit(2, "mm"),
column_gap = unit(2, "mm"),
width = unit(10, "cm"),
height = unit(10, "cm"),
column_title = c("Astrocytic cluster", "Neuronal cluster"),
column_title_gp = gpar(fontsize = 10),
row_title_rot = 0,
show_column_names = F,
show_row_names = F,
row_names_gp = gpar(fontsize = 8),
row_title = c("Astrocytic\n cluster", "Neuronal\n cluster"),
row_title_gp = gpar(fontsize = 10))
my_heatmap = grid.grabExpr(draw(ht))
ht
upper_panel <- ggarrange( Density_plot_of_centralities.panel, my_heatmap, ncol = 2, widths = c(.4, 1), heights = c(1,.3), labels = c('','c'),
hjust = -4, vjust = .8)
upper_panel
Distribution of pairwise correlations
row_order(ht)[[1]] -> astro_cluster
row_order(ht)[[2]] -> neuron_cluster
data$`Node type` -> Nodes
data$ID[neuron_cluster] -> neuron_cluster_names
data$ID[astro_cluster] -> astro_cluster_names
ht@matrix %>% as.data.frame -> heatmap_matrix
heatmap_matrix[neuron_cluster_names,neuron_cluster_names] %>% as.matrix %>% c -> `Neuronal_cluster`
heatmap_matrix[astro_cluster_names,astro_cluster_names] %>% as.matrix %>% c ->`Astrocytic_cluster`
heatmap_matrix[astro_cluster_names,neuron_cluster_names] %>% as.matrix %>% c -> `Neuron_vs_Astrocyte`
data.frame(`Neuronal_cluster`) %>% gather -> A
data.frame(`Astrocytic_cluster`) %>% gather -> B
data.frame( `Neuron_vs_Astrocyte`) %>% gather -> C
quads <- rbind(A,B,C)
colnames(quads) <- c("Comparison","Node correlation")
#quads$Comparison %>% unique()
my_comparisons <- list( c("Neuronal_cluster", "Astrocytic_cluster"),
c("Neuronal_cluster", "Neuron_vs_Astrocyte"),
c("Astrocytic_cluster", "Neuron_vs_Astrocyte") )
ggplot(quads, aes(x=Comparison, y=`Node correlation`, fill=Comparison)) +
geom_violin(trim=F, colour = "azure4")+
stat_compare_means( vjust= -1., hjust= 0, comparisons = my_comparisons, method = "wilcox.test", p.adjust.method = "bonferroni", label = "p.signif")+
scale_fill_manual(values = alpha(c("deepskyblue3", "blueviolet", "brown2"), .8)) +
theme(plot.title = element_text(hjust = 0.5),legend.position="none", axis.title.x=element_blank(),
axis.text.y = element_text(angle = 45, hjust = 1), axis.text.x = element_text(angle = 50, hjust = 1)) +
#ggtitle("Neuronal self-correlations") +
scale_y_continuous(limits=c(-0.7, 2)) + geom_hline(yintercept=0, linetype="dashed", color = "darkred", size=1)-> corr_comparisons
corr_comparisons
library(ProjectionBasedClustering)
Registered S3 method overwritten by 'htmlwidgets':
method from
print.htmlwidget tools:rstudio
library(PCAtools)
Loading required package: ggrepel
Attaching package: ‘PCAtools’
The following objects are masked from ‘package:stats’:
biplot, screeplot
library(magrittr)
Attaching package: ‘magrittr’
The following object is masked from ‘package:purrr’:
set_names
The following object is masked from ‘package:tidyr’:
extract
p <- pca(my.corr.mat)
cbind(p$rotated$PC1, p$rotated$PC2) %>% as.matrix %>% as.data.frame-> my_pca
my_pca %>% cbind( data$`Node type`) %>% set_colnames(c('PC1','PC2','node')) -> to.pca.scatter
summaryzed.optimality -> Op
total.abs.centrality -> Centrality
Centrality -> Ce
b <- ggplot(to.pca.scatter, aes(x = PC1, y = PC2))
b + scale_color_manual(labels = c("Astro", "Exch",'Neu','Si/De Ast', 'Si/De Neu'), values = c("deepskyblue3",'darkgreen','brown2','darkgoldenrod','blueviolet')) +
theme(legend.position="right", legend.box = "vertical")+ geom_point(aes(size = Op, color = node))-> pca_node_RedCosts
b <- ggplot(to.pca.scatter, aes(x = PC1, y = PC2))
b + scale_color_manual(labels = c("Astro", "Exch",'Neu','Si/De Ast', 'Si/De Neu'), values = c("deepskyblue3",'darkgreen','brown2','darkgoldenrod','blueviolet')) +
theme(legend.position="right", legend.box = "vertical")+ geom_point(aes( size = Ce,color = node))-> pca_node_centrality
bottom_panel <- ggarrange( corr_comparisons,pca_node_centrality, pca_node_RedCosts , ncol = 3, widths = c(.4,1,1) , labels = c('d','e','f'),
hjust = 0, vjust = 1)
Removed 3 rows containing non-finite values (stat_ydensity).Removed 3 rows containing non-finite values (stat_signif).Removed 26 rows containing missing values (geom_violin).
ggsave(file="panel_without_graph.png", plot=panel_without_graph, width=13, height=9, dpi = 320)
Node types for Networkx (python) attributes
library(tidyverse)
library(magrittr)
Results <- read_csv("Results.csv")
data_Networkx <- Results%>%
mutate(`Node_type` = ifelse(str_detect(Formula, regex('\\[[a-z]A\\]', ignore_case = T)), 'Astrocyte', NA)) %>%
mutate(`Node_type` = ifelse(str_detect(Formula, regex('\\[[a-z]N\\]', ignore_case = T)), 'Neuron', `Node_type`)) %>%
mutate(`Node_type` = ifelse( str_detect(Formula, regex('\\[[a-z]A\\]|\\[[a-z]N\\]',ignore_case = T), negate = T), 'Exchange', `Node_type`)) %>%
mutate(`Node_type` = ifelse(str_detect(Formula, regex('\\[[a-z]A\\]',ignore_case = T)) &
str_detect(Name, regex('DM_|Demand|sink',ignore_case = T)), 'Sink/Demand Astro', `Node_type`))%>%
mutate(`Node_type` = ifelse(str_detect(Formula, regex('\\[[a-z]N\\]',ignore_case = T)) &
str_detect(Name, regex('DM_|Demand|sink',ignore_case = T)), 'Sink/Demand Neuron', `Node_type`))
data_Networkx %<>% select(c(ID, Node_type))
write_csv(data_Networkx, 'data_Networkx.csv')